A rapid, simple and highly sensitive High Performance liquid chromatography tandem mass spectrometry (LC/MS/MS) method has been developed and validated for the quantitative determination of Azithromycin dehydrate in human plasma. The drug and internal standard was extracted form plasma by liquid-liquid extraction using tertiary butyl methyl ether and n-Hexane (90:10% v/v). The chromatography was carried out by using Qualisil analytical column with 150 x 4.6mm, 5�µ dimension and a mobile phase consisting of Acetonitrile: 20mM Ammonium acetate (70:30 % v/v) the analyte was monitored in Single reaction-monitoring (SRM) mode and the m/z value for azithromycin and internal standard is 749.4 > 591.3 and 734.3 > 567.3 respectively. The lower limit of quantification (LLOQ) was 4.8 ng/ml and the total run time was 3.5 min. A good linear response was observed for the analyte from 4.8 ng/ml to 1577 ng/ml. This method is useful for therapeutic drug monitoring and pharmacokinetic studies.
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